Next Generation Sequencing of lung homogenate mRNA from 8-12 week male C57BL/6 mice following Sham, Carbon Black, Ozone, or Carbon Black-Ozone Co-exposure.

While investigation of a single inhaled toxicant may be useful, the additive effects of toxicants interacting within the body can only be modelled through co-exposure. The purpose of this study is to understand how inhalation of carbon black and ozone alters the transcriptome, and if co-exposure incites unique, genome-wide changes in the lungs. C57BL/6 mice (8-12 weeks old) were exposed to carbon black (10 mg/m3) and/or ozone (2 ppm) for a duration of (3 hours) over a single exposure (Day 1) or four independent exposures (Day 4). Lungs were removed, RNA isolated, and mRNA sequencing performed using the Illumina HiSeq. Libraries were built with the TruSeq® Stranded mRNA Library Prep Kit (Illumina, San Diego, CA) and run on the HiSeq 2500 (Illumina) in 51bp paired end reads. Fastq files were mapped and quantified using the mapping-based mode of Salmon 1.1.0.