Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III [Micro-C seq]

Purpose: This study was designed to determine the mechanism of condensin and Sir2 recruitment to the RDT1 promoter region of S. cerevisiae chromosome III, and to test whether preventing condensin recruitment by deleting the key factors, Fob1 and Lrs4, significantly alters the conformation of chromosome III. Conclusions: Conformational changes in chromosome III and loss of CEN12-rDNA looping in chromosome XII observed in lrs4? and fob1? mutants. Micro-C XL profiles of wild type, lrs4?, and fob1?, strains grown in YPD media were generated.