Clinical and molecular features of the 3 patients investigated by high-throughput sequencing.

Three patients, 2 showing a pattern compatible with a direct evolution and 1 consistent with the existence of a CPC, all having a IgH-VH3 family clonal rearrangement were included in the study.Samples AD1548 and 1787 from pt1, shown in italic, were not included in the high-throughput sequencing analysis of IgH-VH but only in the SNP CNV study.±The sample ID corresponds to the cell suspension vial ID or DNA identification number.P = progression, R = relapse, SD = stable disease; subsequent number relate to episode e.g. R2 = second relapseN.A. = not available SHM = somatic hypermutationFL = Follicular Lymphoma; t-FL = transformed Follicular LymphomaMBR stands for Major Breakpoint Region and mcr for minor cluster region* Pt2 has 2 different mutations at the same base (a pattern compatible with direct evolution)# Samples R8403FL1 and R9129FL2 had 2 different DH/JH rearrangements but when aligned they showed the a similar CDR3 region, with additional 21 insertions in the sample R8403FL1 (see S6 Fig)Clinical and molecular features of the 3 patients investigated by high-throughput sequencing.