We grew wild type and corresponding mutant abf1 and rap1 temperature sensitive yeast at 25 C, then for one hour after shifting to 37 C. We then prepared nucleosomal (mostly mononucleosomal) DNA and hy

NIAID Data Ecosystem20

RNA sequencing of Tcf7 and Prdm1 deficient T cells [RNAseq_Tcf7Prdm1DKO]

T细胞分化

转录因子调控

The transcription factors Tcf7 (encoding TCF1) and Prdm1 (encoding Blimp1) exhibit opposing effects on T cell differentiation. While TCF1 promotes the maintenance of T progenitor exhausted cells, Blim

NIAID Data Ecosystem00

Gene expression profiles of human memory B cells stimulated with BCR/CD40L/IFN-γ/IL21. Gene expression profiles of human memory B cells stimulated with BCR/CD40L/IFN-γ/IL21

细胞基因表达

IL-23信号通路

Analysis of the effect of BCR/CD40/IFN-γ/IL21 on human B cells at gene expression level. Overall design: Total RNA samples were obtained from human memory B cells subjected to 24 hours of in vitro cul

NIAID Data Ecosystem30

CUT&RUN analysis for phospho-MYB and active histone marks in FKBP-MYB Jurkat cells.. CUT&RUN analysis for phospho-MYB and active histone marks in FKBP-MYB Jurkat cells.

转录因子调控

CUT&RUN表观基因组分析

CUT&RUN analysis was performed in Jurkat cells with FKBP knock-in at the MYB locus to analyze DNA bindings of phospho-MYB, H3K27ac, H3K4me3 after dTAG-mediated depletion. Overall design: Jurkat cells

NIAID Data Ecosystem00

Influence of ATL-type Helios overexpression or WT-Helios/Ikaros knockdown on gene expression profile in Jurkat cells

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