Data_Sheet_1_Extraction and Application of Natural Rutin From Sophora japonica to Prepare the Novel Fluorescent Sensor for Detection of Copper Ions.DOCX

Rutin (R), a representative flavonoid found in various biomasses, can be used to prepare different fluorescent sensors for environmental, biological and medical fields. In this work, the natural R in Sophora japonica was extracted and purified to prepare fluorescent-responding sensor systems intended to recognize copper ions with both strong selectivity as well as appropriate sensitivity. Results showed that neat R had no obvious fluorescent emission peak in PBS buffer solution. However, when R and (2-hydroxypropyl)-β-cyclodextrin (CD) were introduced within buffer solution, fluorescent emission intensity was significantly increased due to the resultant R-CD inclusion complex. In addition, the formed R-CD inclusion complex was shown to behave as the aforementioned fluorescent sensor for copper ions through a mechanism of quenched fluorescent emission intensity when R-CD became bound with copper ions. The binding constant value for R-CD with copper ions was 1.33 × 106, allowing for quantification of copper ions between the concentration range of 1.0 × 10–7–4.2 × 10–6mol⋅L–1. Furthermore, the minimum detection limit was found to be 3.5 × 10–8mol⋅L–1. This work showed the prepared R-CD inclusion complex was both highly selective and strongly sensitive toward copper ions, indicating that this system could be applied into various fields where copper ions are of concern.

Rutin（R），一种广泛存在于各种生物质中的代表性黄酮类化合物，可用于制备适用于环境、生物和医学领域的多种荧光传感器。在本研究中，从Sophora japonica中提取并纯化了天然R，以制备旨在识别铜离子的荧光响应传感器系统，该系统具备高度的选择性和适宜的灵敏度。实验结果表明，未经处理的R在PBS缓冲溶液中未出现明显的荧光发射峰。然而，当R与(2-羟丙基)-β-环糊精（CD）引入缓冲溶液中时，荧光发射强度显著增强，这是由于形成的R-CD包合复合物所致。此外，研究表明，所形成的R-CD包合复合物能够作为上述铜离子荧光传感器，其机制在于R-CD与铜离子结合时，荧光发射强度被淬灭。R-CD与铜离子的结合常数值为1.33 × 10^6，可在1.0 × 10^-7–4.2 × 10^-6 mol⋅L^-1的浓度范围内进行铜离子的定量分析。此外，最低检测限为3.5 × 10^-8 mol⋅L^-1。本研究表明，所制备的R-CD包合复合物对铜离子具有高度的选择性和灵敏度，表明该系统可应用于各种关注铜离子的领域。