Purification of acidocalcisomes on iodixanol step gradients.
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Yield values are percentages relative to the 15,000×g pellet fraction and represent averages from number of preparations in parentheses.*Pyrophosphatase activities in the 15,000 g pellet, and the 1st and 2nd gradient acidocalcisome preparations were 0.22±0.09, 15.6±3.2, and 22.6±2.1 µmol min−1 mg−1 protein, respectively (mean ± SD).Purification of acidocalcisomes on iodixanol step gradients.
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2015-12-02
