MAA-SIP biomethanisation with hydrogen addition

Differences in the metabolic behaviour of the microbial community during anaerobic digestion and in situ H2 biomethanation (with and without prior slurry adaptation to H2 addition) was mapped using metagenome resolved protein stable isotope probing (protein-SIP) through incubations of identical reactors with 13C labelled bicarbonate over a period of 48 hours. Detection of labelled proteins was used to evaluate the impact of H2 addition on important anaerobic digestion processes such as syntrophic acetate oxidation, homoacetogenesis, and methanogenesis.