No positive signals for Cx3cl1 or Cx3cr1 mRNA were detected in the brain sections of estradiol-17β (E2)-treated ovariectomized rats hybridized with the corresponding sense probe.
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Supplementary Data. No positive signals for Cx3cl1 or Cx3cr1 mRNA were detected in the brain sections of estradiol-17β (E2)-treated ovariectomized rats hybridized with the corresponding sense probe. (A) Representative single in situ hybridization (ISH) images with Cx3cl1 cRNA antisense or corresponding sense probe. No positive signals for Cx3cl1 mRNA were detected in the brain sections hybridized with the corresponding sense probe. Scale bars, 100 μm. (B) Representative single ISH images with Cx3cr1 cRNA antisense or corresponding sense probe. No positive signals for Cx3cr1 mRNA were detected in the brain sections hybridized with the corresponding sense probe. Scale bars, 100 μm; ARC, arcuate nucleus; VMH, ventromedial hypothalamus.
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2025-08-08



