We performed RNA-seq to compare immune modulators in glutamine-deprived versus glutamine-replete cancer cells. Overall design: RNA-seq was performed on glutamine-depleted and -replete H460 cells in tr
A cell supsension containing an equal mix of HEK and 3T3 cells was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq. Cells were mixed at a final concentration of 12.5 cells
Our previous experiments suggested that HtrA2/Omi could regulate the transcription of certain genes through its PDZ domain. Here, we used an inducible Tet-On system to mimic stress-induced HtrA2/Omi u