Extrachromosomal circular DNA was sequenced in Arabidopsis thaliana and Oryza sativa tissue on a Miseq platform. Fastq files contain 2x250 paired sequencing reads.

Long time considered as « junk DNA », the evolutive force of transposable elements (TEs) is now well established and TEs contribute strongly to eukaryote genome plasticity. However, it is difficult to fully characterize the mobile part of a genome, or active mobilome, and tracking TE activity remains challenging. We therefore propose to use the detection of extrachromosomal circular DNA as a diagnostic for plant TE activity. Our mobilome-seq technique allowed to identify a new active retrotransposon in wild type rice seeds, and will represent a powerful strategy in characterizing the somatic activity of TEs to evaluate their impact on genome stability and to better understand their adaptive capacity in multicellular eukaryotes.