Hemoglobin-induced Transcriptomic Changes in Human Cerebral Arteries

Hemoglobin (Hb) in the subarachnoid space significantly impacts cerebral vessels, leading to various pathological outcomes. The toxicity of cell-free Hb released from erythrocytes and its metabolites is suggested to cause vasoconstriction and neuronal damage, correlating with delayed ischemic neurological deficits (DIND). Here, we aim to describe the changes in the genetic profile of human cerebral arteries exposed to free Hb for 48 hours. We performed an ex vivo treatment followed by mRNA sequencing of the vessels Fresh human cerebral arteries were collected and processed as T0 vessel control for zero or immediate timepoint, T48 vessel control for 48 hours of culturing in media, and Hb vessels for 48 hours in culture with Hb treatment. We have isolated RNA and performed sequencing and compared the transcriptomic changes between the T0 (immediately processed), T48 vessel control and Hb (hemoglobin-treated) groups.