A genome-wide survey of DNA methylation in OsNAR2.1 transgenic plants

DNA methylation is an important mechanism of plant epigenomes, involved in regulating gene expression under environment stress. Nitrogen (N) resource is a significant limiting factor for plant growth and productivity in both natural and agricultural systems. To cope with the flexible environmental N changes, transgenic technolgies have been applied to enhance the N use efficiency (NUE) in crops. Here, we use Whole Genome Bisulfite Sequencing (WGBS) and Methylated DNA Immunoprecipitation Sequencing (MEDIP-seq) to study genome-wide methylation across transgenic plants with higher NUE, transgenic controls and their wild types. Compared with wuyujing 7 (the wild type), the majority of methylation was investigated by WGBS in T0 plants of regeneration from callus(CK), pOsNAR2.1-OsNAR2.1 transgenic high NUE plants (pOsNAR2.1-OsNAR2.1), and false positive plants without gene separated from pOsNAR2.1-OsNAR2.1 transgenic line (FP). We also performed Methylated DNA Immunoprecipitation Sequencing (MEDIP-seq) for wild type of Oryza sativa L. ssp. Japonica cv. Nipponbare (NP), T8 generation plant of knockdown plant of OsNAR2.1 by RNA interference (RNAi) and T8 generation plant of over expression plant of OsNAR2.1 by pUbi-OsNAR2.1 transgenic (pUbi-OsNAR2.1).