HPLC-PDA-MS/MS Data Supporting the Identification of Anthocyanins in Black Carrot Extract for Double-Emulsion Microencapsulation

This dataset contains source chromatographic and mass spectrometric data associated with the study “Tailoring anthocyanin stability with pea–rice proteins and fructooligosaccharides: A microencapsulation study using double emulsion” by Kurek, Pokorski, Aktas, and Custodio Mendoza (2025), published in Food and Bioproducts Processing, Volume 154, pp. 99–107 (DOI: 10.1016/j.fbp.2025.09.002). The associated study investigated the stabilization and microencapsulation of anthocyanins using plant-protein-based systems and water-in-oil-in-water (W/O/W) double emulsions. The data deposited here provide analytical characterization of the anthocyanin material used in the study and complement the physicochemical and microencapsulation results reported in the publication. The dataset includes an anthocyanin profile obtained by HPLC-PDA-MS/MS analysis and a representative HPLC chromatogram recorded using photodiode array (PDA) detection at 516 nm. The analytical data provide retention times, UV–Vis absorption maxima, precursor ions (m/z), and characteristic MS/MS product ions for the anthocyanins identified in the analyzed black carrot extract. Five major cyanidin derivatives are reported: cyanidin 3-xylosylglucosylgalactoside, cyanidin-3-xylosyl galactoside, cyanidin-3-xylosyl(sinapoylglucosyl)galactoside, cyanidin-3-xylosyl(feruloylglucosyl)galactoside, and cyanidin-3-xylosyl(coumaroylglucosyl)galactoside. The accompanying HPLC-PDA chromatogram shows their separation over a retention time range of 0–20 min, with detection at 516 nm. Compound identification was supported by the combination of chromatographic retention behavior, UV–Vis absorption characteristics, precursor-ion masses, and MS/MS fragmentation patterns. These complementary analytical parameters provide evidence for the qualitative identification of the glycosylated and acylated cyanidin derivatives present in the black carrot anthocyanin material. The dataset therefore provides underlying analytical information on the anthocyanin source used in the microencapsulation experiments described in the associated article. It complements the published results concerning anthocyanin stabilization and double-emulsion formulation by documenting the chromatographic and mass-spectrometric characteristics of the anthocyanins subjected to the investigated encapsulation process.