DTU_SRH_001: Virus specific CD8 T cells in 10 healthy donors and 2 hepatitis donors

This study served to verify up to 6 MHC-I-tetramer specificities for each of 12 donors (10 healthy blood donors and 2 hepatitis doors). Virus-derived epitopes were derived from CMV, EBV, VZV, HAdV-C, B19, HIV-1 and VACV along with one MART-1 tumor specifity. Each file is named according to the specific donor, epitope sequence and HLA-restriction used. Each tetramer-specificity was encoded as a combination of 1-2 fluorochromes; a list of combinations is present as a metadata file along with information of percieved reactivity to cognate peptide stimulation in a previous assay (see Pagh Kristensen et al 2023 BioRvix, https://doi.org/10.1101/2023.11.06.565606). This study also performed downsampling and computational analysis of virus-specific populations compared to bulk CD8 T cells from each donor. Maximum 5000 cells were exported from each population including bulk CD8 T cells and used as input for FlowSOM clustering and umap dimentionality reduction, 8 clusters were chosen to represent different phenptypic groups within the dataset.