Topology of TCR signaling networks and effects of LAT deficiency.

(A and B) Phosphorylation-specific networks based on integration of our phosphoproteomic data with protein-protein interactions in the STRING database. Note that both networks show constellation of hubs characterized by interacting proteins function. Zooms into the signaling hub of LAT efficient (LAT+/+) or deficient (LAT−/−) cell lines shows first neighbors (pistachio green or orange circles) of CD3ζ, LCK and ZAP-70 (red or blue circles). (C) The number of edges and (D) degree distribution for experimental and random networks. Orange and pistachio-green triangles correspond to degree distribution in networks based on data from two replicas in Jurkat CL20 cell line. Blue triangles and purple crosses correspond to networks based on the data in LAT-deficient cell line and randomly selected proteins, respectively.