Role of AcsR in expression of the acetly-CoA synthetase gene in Vibrio vulnificus

VarS/A is one of the global factors regulating diverse aspects of metabolism and virulence of bacteria including pathogenic Vibrio spp. An experiment to identify VarS/A-regulon in V. vulnificus revealed that a putative LuxR-type transcriptional regulator was down-regulated in ΔvarA mutant. To investigate the roles of this regulatory cascade from VarS/A to a LuxR-type regulator in V. vulnificus, the target gene regulated by a LuxR-regulator was identified and its expression was characterized. Four-condition experiment, Mo6 vs acsR, Mo6 vs VarA. Biological replicates: 9 wild-type strain, 4 acsR deletion mutant strain, 5 varA deletion mutant strain, independently grown and harvested. Please note that 'wild-type Mo6 vs acsR mutant replicate 1' sample was hybridized to the 30K platform chip while the others were hybridized to the 15K platform Therefore, the 'MO6 vs acsR_1.gpr' has 30,755 data rows (while the other files have 16,355). The 30K platform chip is made of duplication of the 15K platform chip with the same probes.