20240305 Gray Figure S3 data

<b>FIG S3 Deletions of copper and nickel exporters have small effects on </b><b><i>E. coli</i></b><b> sensitivity to HOSCN in the presence of their respective metals. </b><i>E. coli</i> strains MG1655, MJG1759 (MG1655 ∆<i>copA767</i>::<i>kan</i>⁺), MJG2344 (MG1655 ∆<i>zntA724</i>::<i>kan</i>⁺), and MJG2346 (MG1655 ∆<i>rcnA731</i>::<i>kan</i>⁺) were grown in MOPS minimal medium without micronutrients containing 0.2% glucose, supplemented with (A) 1 µM CuCl₂, (B) 1 µM ZnSO₄, or (C) 100 µM NiSO₄ and/or 400 µM HOSCN, as indicated, incubating at 37°C with shaking and measuring A₆₀₀ at 30-minute intervals for 24 hours (n=3-4 experimental replicates, with 2-3 technical replicates per experiment; error bars of 1 standard deviation).