Transcriptomes of de novo lab-adapted monomorphic Trypanosoma brucei (EATRO 1125) and naturally monomorphic T. evansi and T. equiperdum.

Here, we produced a series of monomorphic trypanosome stocks, creating snapshots of the transition from pleomorphism to monomorphism, by continuous rapid syringe-passage in mice using a pleomorphic T. brucei. We then compared the transcriptomes of these artificial monomorphic trypanosomes, alongside several naturally monomorphic T. evansi and T. equiperdum strains, with the pleomorphic T. brucei, to analysis the causes of the monomorphism. Trypanosomes grown in mice were harvested at different stage post infection depend on strains (Detailed information was descrided in overall design), and mRNA profile of each sample was generated by deep sequencing, in triplicate. Three replicates each of three conditions (ascending A/slender, ascending B/stumpy, ascending C/less stumpy) of pleomorphic T. brucei (Tbp1) were analysed, but only two replicates isolated from 'Ascending B' ( Tbp1B) were qualified for subsequent analysis. 'Ascend A' samples were derived from a pool of three infections , to generate sufficient material for analysis. 'Ascending B' and 'Ascending C' samples were each derived from infections in three individual mice, . All samples were analysed for their cell cycle status by scoring the number of kinetoplasts and nuclei in parasites and the presence of morphological stumpy forms, as well as the sensitivity to differentiated stimulus signals. Three replicates each of two conditions (ascending A(low parasitemia), ascending C(peak parastmia)) of passaged monomorphic T. brucei (Tbp40) were analysed. 'Ascend A' samples were derived from a pool of three infections , to generate sufficient material for analysis. 'Ascending C' samples were each derived from infections in three individual mice. Three replicates each of one condition (ascending C(peak parastmia)) of passaged monomorphic T. brucei (Tbp80, Tbp120) were analysed. 'Ascending C' samples were each derived from infections in three individual mice. T. evansi and T.equiperdum samples were each derived from infections in three individual mice.