OMAP-5 Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Liver with SIMS, v1.3

OMAP-5 was designed for Secondary Ionization Mass Spectrometry (SIMS) imaging of fresh frozen human liver samples<br>(https://doi.org/10.1101/2022.09.26.508878). The panel contains 20 antibodies and a cationic nucleic acid intercalator<br>that contains natural-abundance iridium. An antibody directed against Na/K ATPase was used for cell segmentation and<br>image alignment. This OMAP provides spatial context for the anatomical structures, metabolic zonation, and cell types<br>present in the [ASCT+B liver table v1.0](https://doi.org/10.48539/HBM476.BQCC.574). This OMAP does not yet contain<br>antibodies for NK cells, dendritic cells, portal fibroblasts, MAIT cells, gamma delta T cells, and hepatic oval cells.<br>Antigens were detected using lanthanide tagged primary antibodies where the metal tags were detected by specific mass<br>channels using C60 SIMS. Details on the antibody staining method can be found here<br>(dx.doi.org/10.17504/protocols.io.b5qmq5u6). Image registration and cell segmentation were performed using Deepcell<br>(Keren et al.) Cell segmentation was performed using nuclear and membrane marker channels. Integrated protein<br>expression was used to determine thresholds for cellular classification. An antibody directed against EGFR was<br>originally included in OMAP-5 v1.0 and v1.1, but was removed due to inconsistent labeling.