Mechanical performance of hybrid polymer-lipid vesicles with leaflet asymmetry engineered using microfluidics

Lipid vesicles consist of aqueous cores surrounded by a bilayer of phospholipids. Hybrid polymer–lipid vesicles incorporate both polymers and lipids, offering promising properties for developing pharmaceuticals, biosensors, and artificial cells. The hybrid vesicles can be symmetric, with two leaflets of identical compositions, or asymmetric, with leaflets of dissimilar compositions, which can lead to dramatically altered properties. However, existing methods for producing symmetric and asymmetric hybrid vesicles often result in heterogeneous compositions and sizes, making it challenging to quantify the effect of asymmetry and limiting applications. Here, we use a microfluidic approach to produce hybrid vesicles with either symmetric or asymmetric leaflets and precisely engineered compositions. We find that the vesicles with asymmetric leaflets are significantly stiffer and tougher than those with symmetric leaflets; moreover, the lateral diffusivity of lipids is greatly decreased. The s..., , # Data from: Mechanical performance of hybrid polymer-lipid vesicles with leaflet asymmetry engineered using microfluidics

Dataset DOI: [10.5061/dryad.cjsxksnmm](https://doi.org/10.5061/dryad.cjsxksnmm)

README: Mechanical performance of hybrid polymer–lipid vesicles with leaflet asymmetry engineered using microfluidics\
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These data support the findings of the article:\
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Huang Y., Manafirad A., Matoori S., Arriaga L.R., Sun S., Chen A., Dinsmore A.D., Mooney D.J., Weitz D.A.\
“Mechanical performance of hybrid polymer–lipid vesicles with leaflet asymmetry engineered using microfluidics”\
Proceedings of the National Academy of Sciences (PNAS).\
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Article link:\
[https://www.pnas.org/doi/10.1073/pnas.2516407123\\](https://www.pnas.org/doi/10.1073/pnas.2516407123\\)

#### Description of the data

This dataset (pnas_dataverse_01232026.zip) contains processed measurements used to generate the figures and statistical analyses reported in the study. The data include vesicle size measurements..., ,