16S rRNA gene V3V4 sequencing data Jangir, Lemos Rocha et al., 2026

Fecal samples from uninfected SPF and LCM mice were sampled, processed, and stored in glycerol as described above. Genomic DNA isolation, 16S rRNA gene amplification and sequencing were performed by Zymo Research Europe using the 16S Targeted Sequencing service. Briefly, DNA was isolated from feces using the ZymoBIOMICS-96 MagBead DNA Kit (Zymo Research, Irvine, CA) and the targeted library preparation was performed with the Quick-16S™ Primer Set V3-V4 (Zymo Research Europe, Freiburg, Germany). Sequencing was carried out on the Illumina MiSeq platform. Unique amplicon sequences were inferred using the Dada2 pipeline31 and the taxonomy assignment was performed using Uclust from Qiime (v.1.9.1)32 with the Zymo Research Database, an internally curated 16S database, as the reference.