Exosomal delivery of FTO confers gefitinib resistance to recipient cells through ABCC10 regulation in m6A dependent manner

In our study, RNA-seq on serum-derived exosomes from NSCLC patients with clinically significant differences in gefitinib sensitivity was performed to explore the differences in serum exosomal RNA expression profiles between the two groups. The exosomes were isolated and the saucer shaped round vesicles of 40–200 nm in diameter were observed with TEM and nanoparticle tracing system. Then, RNA-seq was performed on serum-derived exosomal RNAs and bioinformatics analysis was performed to analyze the differentially expressed RNAs between GS and GR group. The results demonstrated that there were 289 mRNAs with significant differences between the two groups, 252 of which were significantly up-regulated and 37 significantly down regulated (GR vs GS) . Serum exosomal RNA expression profiles of gefitinib resistant-Exo (GR) and gefitinib sensitive-Exo (GS) groups.