K562 Chemical arrest cell cycle phase fractions 2D-MS-CETSA

K562 cells were synchronized by double thymidine block(DTB) based chemical arrest scheme into G1/S, S and Prometaphase(PM) fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC and 57ºC), (Figure 1A, Methods section of the associated paper), with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on Mass Spectrometer.
The labeling order of samples in the TMT10 channels is as follows:
37C/47C/50C/52C/54C/57C samples: Biorep1_G1S(126), Biorep1_S(127N), Biorep1_PM(127C), Biorep2_G1S(128N), Biorep2_S(128C), Biorep2_PM(129N), Biorep3_G1S(129C), Biorep3_S(130N), Biorep3_PM(130C), mixed_control(131)