Mitochondrial Fission 1 (FIS1) knockdown effect on the H3K27Ac status of leukemia cell line MOLM-13 cells

To examine if loss of FIS1 would alter the H3K27Ac status of leukemic cells, we used shRNA to knock down the expression of FIS1 in leukemic cell line MOLM-13 cells and performed H3K27Ac CHIP-seq to characterize any changes in the H3K27Ac marks upon loss of FIS1. Expressin of FIS1 is knocked down by shRNA in leukemic cell line MOLM-13 cells, technical triplicates are performed. Control and knock-down cells are harvested and subjected to H3K27Ac CHIP-seq analysis.