LC-MS to identify Deer Bone Hydroethanolic Extract (DBHE)

We applied LC-MS to identify the proteins and peptides of DBHE. Protein and peptide identification was achieved through a searchable database. The database utilized for this purpose was the Canadian deer protein database, which was downloaded from NCBI [Cervus_canadensis_GCF_019320065.1_protein.faa]. The software employed for searching was MaxQuant version 2.1.4.0. Gene Ontology and KEGG Pathway enrichment analysis were conducted using the EBI database and InterProScan software version 5.31-70.0.