RNA-sequencing of bovine fetal ovaries throughout gestation

Understanding the developmental origins of reproductive disorders cannot be achieved without understanding normal development and the connected transcriptomics of the fetal ovary. To better understand this, we profiled and analysed RNA from fetal ovaries across gestation (62-276 days, n=19). The platform used detected 25,133 genes for each sample across gestation. Principal Component analysis of the data clustered the samples in three stages of development [early I (up to 98 days), early II (98-135 days) and late (> 135 days)]. Differential expression of genes between the three stages will then be used to delineate the pathways which are up-/down-regulated during these developmental stages. Bovine fetal ovary mRNA profiles from two gestational stages, early (≤ 135 days, n=9) and late (> 136 days, n=10)