The bumblebee Bombus terrestris carries a primary inoculum of Tomato brown rugose fruit virus contributing to disease spread in tomatoes

<b>Fig 3. </b><b>ToBRFV contaminates bumblebee hive components.</b> <b>a.</b> Internal bumblebee hive components. <b>b.</b> ToBRFV detection in individual bumblebees from beehives A, B, C, N by RT-PCR analysis using F-5476 and R-6287 primers. <b>c.</b> ToBRFV detection in comb and cotton from beehives A-C by RT-PCR using F-1 and R-1572 primers. <b>d.</b> ToBRFV detection by RT-PCR in comb and cotton from a naive beehive using F-1 and R-1572 primers. <b>e,f.</b> Detection of ToBRFV coat protein in beehive components by Western blot analysis. N, naïve beehive; M, molecular size marker; W, water control; *, amplicon subjected to Sanger sequencing; D1, a bumblebee from contaminated beehive D; v, viral extraction; CP, coat protein.