A modular dCas9-based recruitment platform for combinatorial epigenome editing
收藏Research Data Australia2024-12-14 收录
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https://researchdata.edu.au/a-modular-dcas9-epigenome-editing/3381264
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资源简介：
Chromatin immunoprecipitation sequencing (ChIP-seq) for histone modifications H3K4me3, H3K9me3 and H3K27me3 in HEK293T cells stably expressing dCas9-SSSavi and 6x sgRNA, and transfected with either DNMT3A+KRAB+EZH2 (D3A-K-E) or aGCN4-mCherry (non-catalytic control) for 72h, followed by flow cytometric enrichment of 200,000 cells positive for GFP (containing SSSavi components).
WGBS was performed on HSB6G cells transfected with either D3A-K-E or GCN4-mCherry for 72h, followed by flow cytometric enrichment of 100,000 cells positive for GFP (containing SSSavi components). Genomic DNA was extracted and processed as detailed in the protocols section.
提供机构：
The University of Western Australia
