Ovarian transcriptome associated with reproductive senescence in the long-living Ames dwarf mice

The aim of the current work was to evaluate the ovarian transcriptome in Ames dwarf (df/df) mice. df/df mice have a delayed ovarian aging compared to normal (N) mice. Although a high number of genes were differentially expressed during aging of N mice, only a small fraction of these changed with aging in df/df mice. These alterations involved more than 500 categorized biological processes. The majority of these biological processes, including inflammatory/immune responses, were up-regulated with aging in N mice, while old df/df mice were characterized by down-regulation of these same processes in comparison to age matched N mice. However, biological processes related to DNA damage and repairing were commonly down-regulated with aging in both genotypes. In conclusion, delayed ovarian aging in long-living df/df mice was associated with reduced expression of genes related to the inflammatory and immune responses. Ovarian mRNA profile of 6 and 22 month old Ames dwarf (df/df) and Normal (N/df) female mice. Twenty mice were used in this study, for each age/genotype 5 ovarian samples were processed. PRJNA327085; SRP077508本研究旨在评估Ames侏儒（df/df）小鼠的卵巢转录组。df/df小鼠相较于正常（N）小鼠，卵巢衰老进程更为迟缓。尽管正常小鼠衰老过程中有大量基因呈现差异表达，但其中仅有极少数在df/df小鼠中随衰老发生表达变化。这些表达改变涉及500余种已分类的生物学过程。在正常小鼠中，绝大多数此类生物学过程（包括炎症/免疫应答）随衰老而上调；而与同龄正常小鼠相比，老年df/df小鼠的上述生物学过程则呈现下调趋势。不过，两种基因型小鼠的衰老过程中，与DNA损伤及修复相关的生物学过程均普遍下调。综上，长寿的df/df小鼠卵巢衰老延迟，与其炎症与免疫应答相关基因的表达降低相关。本数据集包含6月龄和22月龄的Ames侏儒（df/df）与正常（N/df）雌性小鼠的卵巢mRNA表达谱。本研究共使用20只小鼠，每个年龄/基因型组均处理5份卵巢样本。研究编号：PRJNA327085；SRP077508