S1 File

Amplification of 16S rDNA was carried out by polymerase chain reaction (PCR) using a thermal cycler (Mycycler, BioRad). The PCR was carried out with approximately 100 ng of pure genomic DNA. The forward primer PA (AGA GTT TGA TCC TGG CTC AG) and the reverse primer PH (AAG GAG GTG ATC CAG CCG CA), were used for amplification of nearly entire gene. The amplification reactions were performed in a 50 μl volume, by mixing 2 µl of template DNA with 2 μl of each primer, 0.66 μl of (3u/µl) Taq polymerase (Genei, India), 1 μl dNTPs (10 μM each dATP, dCTP, dTTP and dGTP) (Genei, India), PCR buffer (10X) 5 μl and 37.34 μl MQ water for each reaction mixture. PCR amplified DNA was purified by HiYield PCR DNA mini kit from Real Biotech Corporation (RBC, India) through gel excision method. Purified DNA was sequenced by Amnion Biosciences Pvt. Ltd, India.