Trypanosoma cruzi isolates of all DTUs (TcI-TcVI) and other trypanosome species, and their respective sequences of cruzipain and homologous genes determined in this study or retrieved from data banks.

aTCC, Code number of the isolates/strains cryopreserved in the Trypanosomatid Culture Collection (TCC); Sequences from cruzipain and homologous genes:bwhole genes,ccatalytic domains;dcruzipain sequences obtained by sequencing of PCR-amplified genes or from genome databases: GenBank, TriTrypDB and drafts genomes from our Tree of Life project or from the Washington University School of Medicine project*;elineages of T. rangeli defined by Maia da Silva et al. [22], [61].