Peptides of ROP5 isoforms identified by MS after GST-Irga6 pull-down.

The first three sections (ROP5A, ROP5B, and ROP5C) show peptides that specifically discriminate between the three ROP5 isoforms. Residues in bold type are unique for the specific isoform indicated in the table. Sections 4 (ROP5A+ROP5C) and 5 (ROP5B+ROP5C) show peptides that fit to both indicated isoforms only. The peptides displayed in Section 6 (ROP5A+ROP5B+ROP5C) show peptides that match to all isoforms except that marked with *, which is found in all ROP5 isoforms but ROP5C1. Altogether 51 peptides were found for ROP5C comprising a sequence coverage of 86.2%, 47 peptides for ROP5B comprising a sequence coverage of 70.3%, and 34 peptides for ROP5A comprising a sequence coverage of 55.9%. In total 65 individual peptides for RH-ROP5 were found. Many of those were limited digests of the ones presented in the table and are not shown. The next best hit for any T. gondii protein yielded 17 peptides. All together 44 additional T. gondii peptides were recovered from this band.