PCR array analysis of the heart of Wild Type (WT) and Ebi3 deficient mice infected for 7 days with Trypanosoma cruzi. Mus musculus

WT and Ebi3 deficient mice were intraperitoneally infected or not with 1,000 forms of trypomastigotes of T. cruzi Y strain for 7 days, and fragments of the heart tissues of non-infected and infected animals were obtained for RNA extraction. Samples were examined by qPCR assay according to RT²ProfilerPCRArrayTM kit of Mouse Innate Immune Responses & Adaptive (PAMM 052Z - Qiagen) on the ABI StepOnePlusTM equipment. Overall design: PCR array gene expression profiling. Heart fragments from five animals of each group were used, and the amount of RNA was normalized prior to gene expression analysis