Activities towards β- and δ-HCH of the ten known naturally occurring LinB variants (organised according to the three sequence-based groups recognised by Lal et al [2], the thirteen synthetic mutants made herein, and the eight variants analysed by Ito et al [14].

The LinBB90A is used as a reference sequence. All generation one (G1) and generation two (G2) mutants were derived from LinBB90A, except G1.7 and G1.8 which were derived from LinBSS04-3 and LinBNM05, respectively. Activities determined herein are given as turnover numbers (±standard deviation) (min−1) and those from Ito et al [14] as specific activities (mM−1 min−1). C indicates variants whose expression required the use of chaperones (see Material and Methods). ND = not determined. 0* = <1 min−1.