Zscan4 binds nucleosomal microsatellite DNA and protects mouse two-cell embryos from DNA damage [ChIP-seq]

Genome-wide binding analysis of Zscan4 showed sequence-specific occupancy at (CA)n microsatellite repeats in 2C-like cells. Genome-wide occupancy profiling of FACT subunit SSRP1 in curaxin-treated mouse ESC demonstrated re-localization from transcription start sites to Zscan4 binding sites. Overall design: We profiled SSRP1 binding sites by performing ChIP-seq using an SSRP1 antibody in control or curaxin-treated mouse ESC. There are twelve samples analyzed and two replicates for each treatment. We also identified direct targets of Zscan4 in 2C-like cells by performing ChIP-seq using a GFP antibody in 2C-like cells (GFP positive population) from the Zprom::GFP-Zscan4 reporter line and control Zprom::GFP line, and profiled genomic occupancy of endogenous Zscan4 using a Zscan4 antibody in the Zprom::GFP line (GFP positive population and control GFP negative population). There are eight samples analyzed.