The Effect of ATRA on survival in ARPE-19 cells-.tif

ATRA caused a low level of apoptosis (<10%) at concentrations from 10^-9 to 10^-6mol/l, but the cell morphology remained normal. The cells were inhibited at the highest concentration of ATRA treatment 10^-5mol/l (Figure 1A). At both 24 h and 48 h, the cell proliferation was inhibited by ATRA in a dose-dependent manner. A small reduction (<10%) in the number of cells also occurred with a lower ATRA concentration of 10⁻⁶ mol/l, but these changes were not statistically significant (P>0.05). When the ATRA concentration was 10^-5mol/l, the cell survival rate was below 50% at both time points (Figure 1B).