Protein Arginine Methyltransferase 1 is Essential for the Meiosis of Male Germ Cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE176443
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Methods: Prmt1 floxed mice were crossed with Rip2-Cre and Pdx1-CreERT2 mice to generate Prmt1 KO and Prmt1 iKO mice. R26-eYFP mice were crossed for lineage tracing experiments and cell sorting. All mice were backcrossed and maintained on a C57BL/6J background. Cre recombination for CreERT2 was induced by total 5 times intraperitoneal injections (75 mg/kg) of corn oil dissolved tamoxifen (T5648, Sigma-Aldrich) over 2 weeks. mRNA profiles of wild type (WT) and Prmt1 -/- mice were generated by deep sequencing, in duplicate, using Illumina Hiseq.
创建时间：
2021-08-12
