Binding of selected aptamers to different cell lines.

A threshold based on the fluorescence intensity of FAM in the flow cytometric analysis was chosen so that 95% of cells incubated with the FAM-labeled unselected DNA library would have lower fluorescence intensity than the threshold. When the FAM-labeled aptamers were allowed to interact with the cells, the percentage of cells with fluorescence above the set threshold was used to evaluate the binding capacity of the aptamer to the cells. 0–10%,-; 11–30%, +; 31–50%, ++; 51–70%, +++; 71–85%, ++++; >86, +++++*Cell lines: Liver cancer (HepG2), normal liver epithelial cell (THLE-2), lung cancer (squamous carcinoma H226 and adenocarcinoma A549), cervical cancer (HeLa), breast cancer (MCF-7, MDA-MB-231), ovarian cancer (TOV-21G), pancreatic cancer (PL45), leukemia (Ramos, CCRF-CEM).Binding of selected aptamers to different cell lines.