Differential gene expression profile of S100A4 null mouse lens

Whole lens transcriptome profiling by RNA-seq analysis was performed to investigate the role of S100A4 in lens architecture and function. These studies yielded the intriguing discovery that absence of S100A4 in the lens results in robust induction of gene expression of S100A5, an olfactory sensory neuron specific protein, and of genes encoding various photoreceptor and Muller glial cell proteins Two biological repeats of wildtype and S100A4 null mouse lens samples (derived from one month-old mice and for each sample, 6-12 lenses were pooled) were evaluated for changes in gene expression by RNA-seq analysis