Occurrences of Potentially Pathogenic Bacteria Along a River That Flows from Forest to Agricultural and Urban Areas

One of the major challenges is the low concentration of the target bacteria in river water. In this study, we applied the coagulation and foam concentration method to obtain DNA from water samples collected from upstream to near the estuary. Obtained DNA was eluted by DNeasy PowerWater Kit. The DNA samples were analyzed by 16S rRNA gene sequencing to clarify the microbial community shifts and to identify potentially pathogenic bacteria. Based on the 16S rRNA gene amplicon sequencing, potentially pathogenic bacteria were detected with greater than 0.1% of their relative abundances.