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Kinetic parameters of unphosphorylated CKβ, phosphorylated CKβ, and phosphorylation-mimic mutant of CKβ.

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https://figshare.com/articles/dataset/Kinetic_parameters_of_unphosphorylated_CK_phosphorylated_CK_and_phosphorylation-mimic_mutant_of_CK_/3308128
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The values (±SEM) of Vmax and Km were obtained from triplicate measurements. All activities were determined by the standard PK-LDH-dependent coupled-enzyme assays as described in the experimental procedures. For the ATP data, the phosphoryl acceptor substrate was choline at 4 mM concentration.
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2016-05-06
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