Observed cuticular hydrocarbons and putative triacylglycerides (TG) in the anogenital regions of male D. mojavensis and D. arizonae detected by direct UV-LDI-o-TOF mass spectrometry, C22 to C34.

B. IBID, anogenital region cuticular hydrocarbons and TGs detected, C35 to C50.1Proposed chemical compositions are listed as the number of carbon atoms followed by the number of double bonds in the hydrocarbon chain; TG: putative triacylglyceride, with preliminary structure supported by electrospray mass spectrometry.2The relative abundance of each CHC species is calculated by dividing the area under the monoisotopic peak by the total area of all CH peaks detected in the same experiment: ++++, >10% of the total area; +++, 5%–10%; ++,1%–5%; +, *The 29 CHCs used in the statistical analyses of D. mojavensis involving the anogenital and ventral abdomen regions.†Compound transferred to females during copulation, see Fig. 5.