soil metagenome Metagenome. soil metagenome

Clubroot disease is a devastating soilborne disease caused by infection with Plasmodiophora brassicae, which primarily affects cruciferous plants. The microbial diversity of the soil is an essential indicator of its quality. This study measured the physicochemical properties of the soil to study the effect of its microbial diversity on the infection of oilseed rape (Brassica napus) with P. brassicae. High-throughput sequences of the soil bacteria and fungi in the inter-root soils of B. napus were analyzed under different treatment conditions. Proteobacteria, Chloroflexi, Rozellomycota, and Basidiomycota are critical phyla in the development of clubroot disease. A principal coordinate analysis showed that the microbial structure in the soil varied substantially in the bacterial community, and there was no significant difference in soil structure in the fungal community. The occurrence of clubroot disease affected the structure of inter-root microbial community composition in the soil, which resulted in a decrease in its community diversity. Simultaneously, the application of the biocontrol bacterium X216 increased the soil microbial diversity. It effectively reduced the occurrence of P. brassicae, and this study provides a basis to study the microbial diversity in mustard greens (Brassica juncea).