RNA-seq profiling of C/EBPβ-overexpressing myoblasts [differentiating myoblasts]

We previously identified C/EBPβ as an inhibitor of myogenic differentiation and its role in muscle satellite cell’s self-renewal and quiescence. C/EBPβ is regulated during the transition from proliferation to differentiation in myoblasts and that overexpression of C/EBPβ inhibits myocyte differentiation. To identify the molecular mechanism by which C/EBPβ inhibits myogenic differentiation, we performed RNA-seq on C/EBPβ-overexpressing differentiating myoblasts. Differentiating C2C12s mRNA profiles of C/EBPβ-overexpressing and empty vector myoblasts.