Analysis of spontaneous rhodopsin gene correction in mouse rod cells.

an is the number of retinas analyzed.
bAverage of spontaneous events per retina, which was calculated by dividing the total number of events by total number of retinas analyzed.
cThe average number of events was corrected for age-dependent loss of rod cells, which occurs at a rate of 33% per year (Figure 3B) [7]. This correction assumes that this rate of loss applies equally to fluorescent and nonfluorescent rods. In Q344X-hRho-GFP/+ retinas, where only single green rods were observed, the correction is simple: at each age the number of green rods was fractionally increased to take into account the expected cell death. For ID2-hRho-GFP/+ mice, a similar correction for single rods was applied; however, in addition, clusters with more than 3 rod cells were assumed not to be affected and clusters with 2 rods were assumed to give rise to singles in an age-dependent manner.
dFrequency per rod cell, based on 64 million rods/retina[42] before decline.
eAssumes relative standard deviation of total counts, xtot used to derive frequency is xtot -1/2., and the relative standard deviation in the frequency is xtot -1/2/xtot.
fCalculated from actual sample variance as where n is number of retinas scored, μ is the mean corrected number of events per retina, and xi is the corrected number of events in each retina i.
Analysis of spontaneous rhodopsin gene correction in mouse rod cells.