Transcriptional comparison of in vitro generated human dendritic cells and peripheral blood circulating dendritic cells.

Classical dendritic cells (cDCs) are rare sentinel cells specialized in the regulation of adaptive immunity. Modelling cDC development is both crucial to study cDCs and harness their potential in immunotherapy. Here we present a novel in vitro cDC differentiation protocol using cord blood CD34+ hematopoietic stem and progenitor cells (HSPCs) co-cultured with bone marrow-derived murine mesenchymal cell line (MS5) engineered to co-express human FLT3L, SCF and CXCL12 (MS5_FS12). Blood cDC1 and cDC2 were isolated from healthy donor buffy coats. In vitro generated cDC1, cDC2 CD206- and cDC2 CD206+ cells were collected at day 14. All cell types were analysed in triplicates with up to 100 cells per sample.