Transcriptome analysis on wild type and mvp mutant zebrafish ventricles at 7dpa by Next Generation Sequencing

In contrast to mammals, the adult zebrafish hearts can regenerate within a short period. Upregulation of major vault protein (Mvp) expression after ventricle resection. To determine the impact of loss of mvp on transcriptome during heart regeneration, we pursued transcriptome analysis (RNA-Seq) on wild type and mvp mutant zebrafish hearts at 7 day-post-amputation. Overall design: We perform ventricle resection or sham surgeries to adult wild type and mvp mutant zebrafish hearts. At 7 days post amputation, ventricle samples were collected in triplicate for RNA-seq.