Bulk transcriptomic analysis of primary beta cells from control, IGT, mild, and severely diabetic mice.

Patients with type 2 diabetes often experience many years of reduced ß-cell function and impaired glucose tolerance preceding diabetes diagnosis. It is postulated that ß-cell function may be compromised by relatively small changes in glycaemia, initiating a gradual decline that underlies diabetes progression. It has been widely reported that chronic hyperglycaemia/diabetes alters the expression of multiple genes involved in glucose metabolism in both rodent and human islets. We therefore investigated the extent to which impaired glucose tolerance (IGT) and chronic mild and severe hyperglycaemia (HG) impact gene expression in mouse ß-cells. Gene expression was measured in primary islets isolated from control, IGT, mild HG and severe HG mice by bulk RNAseq.