Relationship between hemagglutinin stability and influenza virus persistence after exposure to low pH or supraphysiological heating

In this work, we developed a high-throughput assay to measure the HA stability of surveillance isolates by monitoring the pH at which these viruses become inactivated. For four A/TN/09 (H1N1) HA mutants and 73 IAVs of varying subtype, virion inactivation pH was compared to HA activation pH and the rate of inactivation during 55 degree Celsius heating.