Julien Mouysset, Alexandra Deichsel, Sandra Moser, Carsten Hoege, Anthony A. Hyman, Anton Gartner, Thorsten Hoppe (2011) CIL:35147, Caenorhabditis elegans, early embryonic cell. CIL. Dataset

This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of cdc-48 and atl-1 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. Downregulation of atl-1 suppressed the P1 cell division delay phenotype of cdc-48, ufd-1, and npl-4 RNAi embryos, suggesting that the DNA replication checkpoint is responsible for the delayed P1 cell division. The series corresponds to experiments shown in Fig. 2A and Movie 6 of Mouysset et al. Movie is CIL 25633 and original data file is CIL 35147.

本时序影像系列展示了经过RNA干扰去除cdc-48和atl-1的秀丽隐杆线虫（C. elegans）胚胎在四细胞期之前的早期分裂过程。P0受精卵的第一次分裂在秀丽隐杆线虫胚胎中是不对称的，产生了一个位于前端的AB细胞和一个较小的后端P1细胞。这些细胞在发育命运和分裂时间上存在差异，其中AB细胞在大约2分钟前于P1细胞进行分裂。时序微分干涉对比（DIC）显微镜观察到，cdc-48、ufd-1和npl-4的下调增加了与AB细胞相比P1细胞的分裂延迟，导致了三细胞期的延长。atl-1的下调抑制了cdc-48、ufd-1和npl-4 RNAi胚胎中P1细胞分裂延迟表型，表明DNA复制检查点负责P1细胞分裂的延迟。本系列影像与Mouysset等人在图2A和电影6中展示的实验相对应。电影编号为CIL 25633，原始数据文件编号为CIL 35147。